Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Regulatory Interplay of Long Non-Coding RNAs in Breast Cancer Progression 1594 1612 225672 10.30491/jabr.2024.470488.1762 EN Vibhushini R Department of Biotechnology, Sri Venkateswara College of Engineering, Post Bag No.1, Sriperumbudur Taluk - 602117, Kancheepuram, Tamil Nadu, India Jyothishree Veerabhadran Department of Biotechnology, Sri Venkateswara College of Engineering, Post Bag No.1, Sriperumbudur Taluk - 602117, Kancheepuram, Tamil Nadu, India 0009-0001-6099-2182 Nalinkanth Veerabadran Ghone Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Rajiv Gandhi Salai (OMR), Kalavakkam - 603110, Tamil Nadu, India 0000-0002-0968-4790 Hariharan Jayaraman Department of Biotechnology, Sri Venkateswara College of Engineering, Post Bag No.1, Sriperumbudur Taluk - 602117, Kancheepuram, Tamil Nadu, India 0000-0002-2545-4661 Journal Article 2024 07 29 Globally, breast cancer is a severe and diverse disease with a high morbidity and death rate. Recent studies have highlighted the critical role of long non-coding RNAs (lncRNAs) play in the initiation and spread of breast cancer. lncRNAs are crucial regulators of gene expression and are implicated in various cellular processes, including tumorigenesis. The dysregulation of lncRNAs has been linked to aberrant cell proliferation in breast cancer. By modulating gene expression at transcriptional and post-transcriptional levels, lncRNAs can promote or suppress cell proliferation, influencing tumor growth and progression. lncRNAs can control the invasion and migration of breast cancer cells, thereby promoting the spread of metastatic cancer to other organs, through interactions with miRNAs and other molecular pathways. Understanding the intricate mechanisms by which lncRNAs contribute to these processes is essential for developing targeted therapies and improving patient outcomes. The crosstalk between lncRNAs and other intracellular proteins is important for understanding their functional roles in breast cancer progression. Protein interactions influence the stability, activity, and localization of lncRNAs, which in turn influences how these RNAs affect cellular processes. This review investigates the complex interactions of lncRNAs with miRNAs and proteins in breast cancer, with a focus on their roles in metastasis, proliferation, and other stages of the disease's progression. Moreover, with the ability to target specific genes, identifying novel biomarkers, therapeutic targets and potential in overcoming drug resistance lncRNAs represent an exciting area in RNA-based therapies and have the potential to transform breast cancer treatment.

https://www.biotechrep.ir/article_225672_57db8f810f92fa2da22b87730a0afbf8.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Optimization of Extracellular Lipases Production by Fungal Strains Isolated from the Soil Enriched with the Oil of Pistacia lentiscus 1613 1621 225674 10.30491/jabr.2024.477960.1778 EN Nouha Ouartsi Laboratory of Microbiology and Molecular Biology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Bourzama Ghania Laboratory of Microbiology and Molecular Biology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Sarah Benouagueni Laboratory of Microbiology and Molecular Biology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Laboratory of Biochemistry and Applied Microbiology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Yousra Benhassine Laboratory of Biochemistry and Environmental Toxicology, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Houria Ouled-Haddar Laboratory of Molecular Toxicology, University of Jijel, Jijel, Ouled Aïssa 18000,, Algeria Sara Sahli Biochemistry Department, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Lamia Hamaidi Biochemistry Department, Badji Mokhtar Annaba-University. 12, P.O. Box, 23000 Annaba, Algeria Journal Article 2024 09 12 Introduction: Lipases are enzymes that catalyze a large number of hydrolysis reactions leading to a vast diversity of industrial applications. Microbial lipases constitute an important group of biotechnologically valuable enzymes, mainly because of the versatility of their properties and ease of their production and extraction. The aim of this research work is the isolation of lipolytic fungal strains from the soil enriched with the oil of Pistacia lentiscus in the Annaba region of Algeria. Materials and Methods: Fungi isolation was carried out on Potato Extract Agar (PDA) at 28 °C. Lipase activity was determined through the estimation of free fatty acids by titrimetry, optical density and final pH. The optimization of lipase production was achieved for several factors, namely, agitation, pH, temperature, biomass, carbon source, lipidic substrate, and time. Results: Three strains were isolated and identified as Aspergillus sp., Fusarium sp., and Penicillium sp., with the latter exhibiting the highest lipolytic activity. The optimization indicated that these strains were able to hydrolyse different fatty substances with high activity under different conditions. Conclusions: The obtained results show that pistachio oil was considered a novel inducer of the lipase-producing fungal strains.

https://www.biotechrep.ir/article_225674_8e10e9ccfbc477794e02584adb84d9b5.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 An analysis of the Dimensions and Risk Management Strategies for the Biotechnology 1622 1630 225677 10.30491/jabr.2024.481455.1789 EN Mehdi Rahmani Department of Knowledge and Information Science, Faculty of Educational Sciences and Psychology, University of Isfahan, Isfahan, Iran 0000-0002-3207-2354 Journal Article 2024 10 02 Introduction: Despite the expansion and development of the field of biotechnology, concerns persist regarding the risks and dangers associated with this sector. The aim of the current research was to prioritize and manage the risks in Iran's biotechnology sector. Materials and Methods: The Meta Synthesis Model (qualitative), Kappa coefficient calculation, and Delphi technique (quantitative) were utilized in this study to identify biotechnological risks. The research encompassed all articles published in English from 2012 to 2022. The Delphi technique engaged experts in biotechnology who were well-versed in risk management. To determine the study population, all researchers in relevant subject areas were identified, and four were selected through purposive sampling. Results: Based on published sources, 56 risks were identified in the field of biotechnology. Using the Shannon entropy method and evaluating the frequency of these risks, 34 were classified as high-priority. The findings indicated that the most significant challenge in biotechnology pertains to the risks associated with human exposure to products contaminated by toxins and biotechnological pollution, which could potentially enter the human body. Therefore, human health emerged as the most critical risk. Furthermore, the results from the fuzzy Delphi technique emphasized that the highest-scoring biotechnological risk in Iran involved human exposure to products affected by toxins and biotechnological pollution. Conclusions: Through pairwise comparison of criteria by experts, it was revealed that challenges related to water and marine resources are among the most important biotechnological risks, with health and medical issues closely following.

https://www.biotechrep.ir/article_225677_1f2fd87d46e3643946e5226c53cebc22.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Development and Optimization of LSPR-based Aptasensor for Detection of Vibrio cholerae 1631 1641 225678 10.30491/jabr.2024.472168.1770 EN Zahra Abolghasemi Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran Seyed Morteza Robatjazi Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran Mehdi Zeinoddini Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran 0000-0002-9500-1334 Journal Article 2024 08 07 Introduction: Vibrio cholerae is one of the primary waterborne pathogens that can enter the biofilm phase during its lifecycle, making it difficult to detect. To address this challenge, we have designed a colorimetric aptasensor based on aptamers and gold nanoparticles (GNPs) for the effective management and treatment of this illness. Materials and Methods: The aptamer sequence was selected based on previous work and amplified using PCR with specific primers. The aptamer, which has a strong binding affinity to V. cholerae, was immobilized on the surface of GNPs. Detection was achieved by observing the aggregation of GNPs induced by the target bacteria, resulting in noticeable color changes in the reaction upon the addition of NaCl. To determine the optimal conditions, a unique method based on a Taguchi orthogonal array was used to evaluate critical parameters such as conjugation time, temperature, pH, and aptamer concentration. Results: The optimal conditions for aptamer immobilization were determined to be pH 9, 3 hours for incubation time 10 °C for incubation temperature, and 550 nM for aptamer concentration. Analysis of signal-to-noise ratios showed that temperature and pH levels significantly affect GNP-aptamer conjugation. Under the optimal conditions, a linear calibration relationship was established between the A630/A524 ratio and V. cholerae concentrations ranging from 102 to 107 CFU/ml. The detection limit and time were found to be 6 CFU/ml and 50 minutes, respectively. Conclusions: The developed colorimetric aptasensor demonstrates high sensitivity and specificity for the detection of V. cholerae, making it suitable for the production of rapid diagnostic kits.

https://www.biotechrep.ir/article_225678_b4a4ca74e945b2b9173a273bd51c812d.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Enhanced Production of Thermostable α-Amylase in Bacillus subtilis 1642 1651 225679 10.30491/jabr.2025.487018.1803 EN Askar Kholikov Institute of Microbiology, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan 0009-0001-3587-4731 Muhammad Latif Nazirov Institute of Microbiology, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan Khushnut Vokhidov Institute of Microbiology, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan Alexandr Kachan Department of Molecular Biology, Belarusian State University, Minsk, Belarus 0000-0001-9960-4274 Anatoli N. Evtushenkov Department of Molecular Biology, Belarusian State University, Minsk, Belarus 0000-0002-2755-6979 Akhmadzhan Makhsumkhanov Institute of Microbiology, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan 0000-0002-0082-3766 Journal Article 2024 11 04 Introduction: The increasing industrial demand for thermostable amylases necessitates the exploration of new sources, especially from understudied regions like Uzbekistan, which may offer novel, reliable, and stable enzyme characteristics. The objective of this study was to screen, identify, and characterize bacterial species producing thermostable α-amylase, clone its corresponding gene, and test B. subtilis 168 htr9 mutant hosts for recombinant enzyme overproduction. Materials and Methods: Bacterial isolates with amylase-producing capabilities were selected using a plate assay, and their taxonomy was confirmed through 16S rRNA sequencing. The α-amylase activity was measured using the 3,5-dinitrosalicylic acid method. The B6-1-5-amyL vector was specifically engineered to clone and express the α-amylase gene in expression hosts. Results: This study presents the first isolation and characterization of bacterial strains from the soils of Uzbekistan that produce thermostable α-amylase. Following a screening process, two isolates, 104.K and amyR, displaying high amylolytic activity, were selected and identified as Bacillus licheniformis 104.K and Bacillus velezensis amyR, respectively. The crude α-amylases from both strains exhibited maximum activity at 90 °C and 70 °C, respectively. The α-amylase gene from B. licheniformis 104.K was cloned into a newly developed B6-1-5 shuttle vector. Notably, overexpression of this amylase gene in Bacillus subtilis 168 htr9 ehp241 led to a 1.14-fold and 373-fold increase in amylase activity compared to the B. subtilis 168 htr9 and B. licheniformis 104.K strain. Conclusions: The B. licheniformis 104.K strain was selected for its thermostable α-amylase, and the gene was successfully cloned and expressed in Escherichia coli and B. subtilis 168 strains. These findings hold promise for enhancing the catalytic efficiency of recombinant α-amylase using computational biology methods and promoting B. subtilis 168 for large-scale production of recombinant enzymes.

https://www.biotechrep.ir/article_225679_9753975c61a6f09da56c9ce41edc90d2.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Evaluation of Genetic Variability, Phenotypic Stability and Interrelationships among the Quantitative Traits of Sugarcane under Drought Stress 1652 1667 225680 10.30491/jabr.2024.463598.1747 EN Ramaswamy Manimekalai ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India 0000-0001-9901-5234 Arockia Jain Mary ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India 0000-0001-9503-7073 K Mohanraj ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India Jini Narayanan ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India Ram Vanish ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India K.P Mrudula ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India J. Saranya ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India M. Gokul ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India Rajeev Kumar ICAR- Indian Institute of Sugarcane Research, Lucknow, India Ranjit Singh Gujjar ICAR- Indian Institute of Sugarcane Research, Lucknow, India Selvi Athiappan ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India S. Vasantha ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India Govinda Kurup Hemaprabha ICAR- Sugarcane Breeding Institute, Indian Council of Agricultural Research (ICAR), Coimbatore, Tamil Nadu 641 007, India Journal Article 2024 06 18 Introduction: Drought is a serious abiotic stress that leads to low sugarcane productivity. The complex polyploidy and lack of heritability information for drought tolerance make breeding more difficult. Yield is a polygenic trait influenced by several phenological and physiological factors. Assessing the variability, correlation and heritability of various traits under water deficit conditions is imperative. In the present study, we measured 14 traits contributing to drought resistance and yield under control (well-watered) and drought (water-stressed) conditions. Materials and Methods: Around 14 quantitative traits for a large population of 119 progenies cross BO 91 x Co 775 were evaluated. The agronomic traits, which included 6 morphological traits, germination%, tillers, cane height, internodal length, stalk diameter, and internodes in a stalk; 2 physiological traits, such as total chlorophyll content and chlorophyll fluorescence; and 6 yield-contributing traits, including single cane weight (SCW), number of millable canes (NMC), cane yield, Brix%, Pol%, and commercial cane sugar (CCS)%, were measured for each clone. Results: Our multivariate analysis revealed a significant difference among the treatments, genotypes, and G × E interactions. Path analysis revealed that SCW had a positive direct effect on cane yield. Here, we suggest that the traits of tiller number, total chlorophyll content, SCW, and NMC could effectively evaluate drought-tolerant genotypes. Conclusions: This formulated study with significant findings of phenotypic stability serves as a potential parameter for breeders in selecting genotypes for arid/semiarid regions.

https://www.biotechrep.ir/article_225680_8f0656a72807ba62ef3c7b9916991ce1.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Prognostic Value of Combined SATB2 and Ki67 Expression in Colorectal Cancer Patients: A Retrospective Cohort Study 1668 1677 225681 10.30491/jabr.2025.512767.1855 EN Abdulameer Kareem. Leelo Al-Obaidy Department of Basic Medical Science, Nursing College, University of Al-Qadisiyah, Diwaniyah, Iraq 0000-0003-3100-9828 Hawraa A. Kareem Department of Al-Jawad Oncology Center, Al-Emamain Al-Kadimain Medical City, Ministry of Health of Iraq, Baghdad, Iraq 0000-0002-8061-7347 Yusra Jabbar Hasan Department of Al-Jawad Oncology Center, Al-Emamain Al-Kadimain Medical City, Ministry of Health of Iraq, Baghdad, Iraq 0009-0006-9589-516X Journal Article 2025 03 16 Introduction: Colorectal cancer is the third most prevalent malignancy, with limited prognostic tools due to its heterogeneity. This study examined the simultaneous expression of SATB2 and Ki67 in colorectal cancer patients by immunohistochemistry, with progression-free survival (PFS) and patient demographics. Materials and Methods: The study employed an observational cohort design with a cross-sectional approach, defined as a retrospective-prospective clinicopathological analysis of 128 colorectal cancer patients to examine SATB2/Ki67 immunoreactivity, including variables such as diagnosis duration, progression-free survival (PFS), age, sex, tumor location, grading, histopathological types, and TNM classification. Survival and ROC curve analysis tests were used to determine 46 months as the PFS cutoff and survival criterion. Results: Fifty-six individuals survived PFS, while 72 did not. Patients averaged 54.45 years old, with a 22-67 months PFS, averaging 47 months. Colorectal tubular adenocarcinoma patients had the highest PFS; PFS was lower for rectal cancer patients. SATB2 expression exhibited no significant changes based on gender or patient age; however, female patients showed increased Ki67 expression compared to males. The average expressions of SATB2 and Ki67 were 40.21% ± 29.41% and 25.27% ± 23.16%, respectively. According to TNM staging, 68.9% of patients were categorized in stages I–II, 23.4% in stage III, and 7.8% in stage IV, with survival rates declining as tumor metastasis increased. Conclusions: The study suggested that evaluating SATB2 expression during colorectal cancer treatment could clarify its prognostic importance. SATB2 expression correlates positively with colorectal cancer survival; nevertheless, elevated Ki67 levels negatively impact prognosis, particularly when SATB2 levels are high.

https://www.biotechrep.ir/article_225681_45dcf085daee3148dcfd2b14be6390e8.pdf

Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 12 2 2025 06 01 Extraction of Artemisia aucheri Essential Oils and Evaluation of their Chemical Composition and Antioxidant Activity 1678 1685 225682 10.30491/jabr.2024.416516.1670 EN Ani Boghozian Department of Biochemistry, Payame Noor University, Tehran, Iran 0000-0002-1341-7812 Hamid Reza Shiran Department of Environmental Engineering, School of Environmental, University of Tehran, Iran 0009-0006-5371-6784 Ali Choopani Amiran Oghyanoos Biotechnology Company, Tehra, Iran 0000-0002-9072-5085 Amirhossein Choopani Department of Environmental Science, University of Agricultural Sciences and Natural Resources, Grogan, Iran 0009-0007-5083-5654 Nahid Ghaed Amini Amiran Oghyanoos Biotechnology Company, Tehra, Iran 0000-0002-5760-2972 Journal Article 2023 09 19 Introduction: Plants are an excellent source of biologically active phytochemical compounds that act as antioxidants to combat free radicals. The aim of this study is to chemically analyze the essential oil compounds (EOs) and evaluate the antioxidant capacity of Artemisia aucheri flowers in vitro. Materials and Methods: Artemisia aucheri was collected from the Kashan region of Isfahan Province, Iran. The flower essential oil was extracted using a Clevenger apparatus, and its components were separated and identified using gas chromatography-mass spectrometry (GC-MS). Results: The antioxidant properties of the flower essential oil were evaluated using the DPPH free radical scavenging method and the β-carotene-linoleic acid bleaching test. Data analysis was performed using SPSS 20 software. The major compounds identified in the flower essential oil were camphene (31.05%), β-myrcene (26.18%), and camphor (15.41%). The DPPH assay showed that the essential oil exhibited significant free radical inhibition, while the β-carotene assay used ascorbic acid as a positive control. Conclusions: Research indicates that many factors influence the primary composition of plants, including climatic conditions, soil type, and genetic factors. Due to the hydrophilic nature of the DPPH assay, the IC50 value of the essential oil was higher than that of ascorbic acid, indicating lower activity in this assay. Conversely, due to the lipophilic nature of the β-carotene assay, the IC50 value of the essential oil was lower than that of ascorbic acid, suggesting stronger antioxidant activity in lipophilic environments.

https://www.biotechrep.ir/article_225682_c34d07090ac98aae3c9851cdef24b71e.pdf