Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 4 3 2017 09 01 Electrochemical Behavior of Adult and Fetal Hemoglobin at Gold-coated Magnetic Iron Oxide Nanoparticle 669 674 68968 EN Khadijeh Eskandari Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Hedayatollah Ghourchian Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran Aghdas Banaei Research Institute of Applied Science, Academic Center of Education, Culture and Research (ACECR), Shahid Beheshti University, Tehran, Iran Journal Article 2017 05 24 <span>A feasible and fast method for Adult Hemoglobin (A-Hb) and Fetal Hemoglobin </span><span>(F-Hb) study was developed by immobilization of Hb on gold-coated magnetic iron oxide nanoparticles (GMNPs).The prepared GMNPs composite nanoparticles with 60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The direct electroche- mistry of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corres- ponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (α) for electron transfer between the electrode surface and pro- tein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb biosen</span><span>sors for H O detection. The lifetime of biosensor is more than 2 weeks.</span> https://www.biotechrep.ir/article_68968_8d0d2b57bdc0fa64bc3348ffcfd5d159.pdf