Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 10 2 2023 06 01 In vivo Acute Toxicity, Analgesic Activity and Phytochemical Characterization of Solenostemma argel (Del) Hayne Essential Oil 976 983 174234 10.30491/jabr.2023.385652.1606 EN Daouia Keltoum Benmaarouf Unit for Evaluating the Efficacy of Pharmacological Molecules and Developing Alternative Strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria 0000-0001-7746-8400 Bernard China Sciensano, Belgian Institute of Health, Wystmanstreet 14, 1050 Brussels, Belgium 0000-0001-5717-478X Diana C. G. A. Pinto LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal 0000-0003-4249-7089 Hamza Aliboudhar Organic Functional Analysis Laboratory, Faculty of Chemistry, University of Science and Technology Houari Boumediene, BP 32, El Alia, Bab Ezzouar, 16111 Algiers, Algeria 0000-0003-3061-5543 Soumiya Boulahlib Organic Functional Analysis Laboratory, Faculty of Chemistry, University of Science and Technology Houari Boumediene, BP 32, El Alia, Bab Ezzouar, 16111 Algiers, Algeria Safia Zenia Unit for Evaluating the Efficacy of Pharmacological Molecules and Developing Alternative Strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria Hanane Bensedira Department of Pathology, Beni Messous Hospital, Algiers, Algeria Kheira Bouzid Department of Pathology, Beni Messous Hospital, Algiers, Algeria Meriem H. Ben-Mahdi Unit for Evaluating the Efficacy of Pharmacological Molecules and Developing Alternative Strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria 0000-0003-4202-5364 Journal Article 2023 02 14 Introduction: Solenostemma argel is a Saharan plant used in traditional medicine to cure pain. The present work investigated the quantitative analysis of the composition of the essential oil of S. argel leaves (EOSA) as well as its acute toxicity and anti-nociceptive activity. Materials and Methods: The chemical characterization of EOSA was carried out by GC-MS and NMR. EOSA acute oral toxicity study was performed according to the OECD-420 method. EOSA anti-nociceptive activities were evaluated by acetic acid-induced abdominal writhing test, hot plate test, and formalin test. Results: Twenty components were identified by GC-MS including Linanool (57.10%), terpineol (12.95%), trans-geraniol (12.65%), and nerol (4.67%). The main compound linalool was isolated by NMR. The EOSA at 250 and 400 mg/kg significantly attenuated acetic acid-induced writhing by 72.71 and 92.41%, respectively. Moreover, Ingestion of EOSA at doses of 250 and 400 mg/kg caused a significant and dose-dependent anti-nociceptive effect in both neurogenic and inflammatory phases of formalin-induced licking. EOSA impacts the pain latency in the hot plate test. Conclusions: The results of this study showed that EOSA has an anti-nociceptive effect on central and peripheral pain.

https://www.biotechrep.ir/article_174234_d969f1354f848be1f5998515c0854117.pdf