Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 8 4 2021 12 01 Computational Analysis of Responsive Transcription Factors Involved in Drought and Salt Stress in Rice 406 413 142528 10.30491/jabr.2020.243913.1272 EN Abbas Saidi Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran Zahra Hajibarat Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran 0000-0002-0322-9404 Asadollah Ahmadikhah Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran 0000-0001-5100-9740 Journal Article 2020 08 15 <span class="fontstyle0"><strong>Introduction:</strong> <span class="fontstyle2">Rice is one of the most important crops to more than half the world's population. The reduction of rice yield has been severely influenced by drought and salt stress. The main purpose of this survey was to detect Transcription Factors (TFs) involved in drought and salt stress in rice.<br /></span><strong>Materials and Methods:</strong> <span class="fontstyle2">In this study, microarray data in PlnTFDB and DRTF were taken to evaluate the expression of responsive TFs to drought and salt stress in the growth stages. A comprehensive analysis of responsive TFs were performed containing gene network, expression analysis in different tissues, and detection of Transcription Factor Binding (TFBs) sites.<br /></span><strong>Results:</strong> <span class="fontstyle2">A total of 80 TFs were found differentially expressed (DEGs) under drought and salt stress in rice. Gene Ontology (GO) revealed that biological processes included transcription, regulation of transcription, regulation of RNA metabolic, and RNA metabolic. In addition, some molecular functions such as organic cyclic compound binding, heterocyclic compound binding, DNA binding, and cellular component are enriched in intracellular and nucleus. To survey selection pressure in responsive TFs under drought and salt conditions, Tajima’s and Fu test analysis revealed balancing selection. Analysis of TFBs illustrated that several TFBs namely AP2, bZIP, and MYB/SANT act as basic TFBs linked to abiotic stress responses as well as different growth stages in rice. The current study revealed that most TFs related to histone modification were up-regulated whereas, TFs associated with the regulation of repression/ activation transcription were down-regulated.<br /></span><strong>Conclusions:</strong> <span class="fontstyle2">Our results can provide an insight into the regulatory mechanisms involved in response to drought and salt stress which can aid to improve rice varieties.</span> </span> https://www.biotechrep.ir/article_142528_bb0998b50ad1d50b2dbb4944b1380616.pdf