Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 8 4 2021 12 01 Adipose-Derived Stem Cells Growth and Proliferation Enhancement Using Poly(Lactic-co-Glycolic Acid) (PLGA)/Fibrin Nanofiber Mats 361 369 125491 10.30491/jabr.2020.223551.1199 EN Mohsen Norouzi Department of Biomedical Engineering, Russ College of Engineering and Technology, Ohio University, Athens, Ohio, USA Department of Tissue Engineering, Faculty of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Isfahan, Iran 0000-0002-7150-2716 Mohammad Rafienia Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-6030-5082 Elahe Poorazizi Department of Biochemistry, Faculty of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, Isfahan, Iran 0000-0003-4509-1885 Mohsen Setayeshmehr Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 0000-0001-6044-1279 Journal Article 2020 03 14 <strong>Introduction: </strong>A Synthetic biomaterial, such as Poly Lactic-co-Glycolic Acid (PLGA) with superior mechanical properties, along with a natural polymer such as fibrin, which facilitates cell attachment and enhances biocompatibility, can be used in the production of novel composite tissue engineering scaffolds.<br /><strong>Materials and Methods: </strong>To carry out this study, 10% polymer solutions with different ratios of PLGA: Fibrin, including 10:0, 9:1, 8:2, and 7:3, were prepared and used in the production of aligned and unaligned electrospun nanofiber scaffolds. Human Adipose-Derived Stem Cells (h-ADSCs) were cultured on the scaffolds, and they were characterized using Fourier Transform Infrared Spectroscopy (FTIR), Energy-dispersive X-ray spectroscopy (EDX), Scanning Electron Microscopy (SEM), mechanical, hydrophilic, degradation, water absorption, and biocompatibility tests.<br /><strong>Results: </strong>The obtained scaffolds consisted of homogeneous fibers, without any beads and water droplets. The percentage of porosities and internal correlation of the cavities were not significantly different between aligned and unaligned electrospun scaffolds <span class="fontstyle0">(</span><span class="fontstyle1"><em>P</em></span>><span class="fontstyle0">0.05)</span> and by adding fibrin, these properties improved, while tensile strength and elasticity decreased. All the scaffolds were hydrophobic and the highest and lowest swelling rates belonged to PLGA/30% Fibrin scaffolds and pure PLGA scaffolds (more than 90% and less than 45%, respectively). There is a significant difference in degradation rates between fibrin-contained scaffolds and pure PLGA scaffolds. Moreover, compared to the aligned electrospun scaffolds, the highest degradation rate of unaligned ones was observed.<br /><strong>Conclusion: </strong>Considering the results of SEM and bio-compatibility experiments, the aligned electrospun PLGA/10% Fibrin scaffold with numerous spindle shape h-ADSCs and unaligned electrospun PLGA/20% Fibrin scaffold with many spindle shape cells together with round shape cells are introduced as optimal options. https://www.biotechrep.ir/article_125491_d163ee23124bb9ac71eb27a8de6e1eef.pdf