Baqiyatallah University of Medical Sciences Journal of Applied Biotechnology Reports 2322-1186 8 4 2021 12 01 In Vitro Investigation of the Anti-Diabetic Effects of Imperialine on Beta-TC6 Pancreatic and C2C12 Skeletal Muscle Cell Lines 337 345 125323 10.30491/jabr.2021.125323 EN Massoud Mashhadi Akbar Boojar Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran 0000-0003-0145-5472 Mahdi Mashhadi Akbar Boojar Department of Pharmacology and Toxicology, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran 0000-0002-2002-9332 Yaghuob Firoozivand Department of Pathobiology, Malekan Branch, Islamic Azad University, Malekan, Iran Journal Article 2020 01 12 <span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Imperialine (Imp) is a steroidal alkaloid present as the main active constituent of medicinal herb, </span><span class="fontstyle2"><em>Fritillaria imperialis</em> </span><span class="fontstyle2">with many biological and therapeutic effects. However, it has not been investigated in vitro for hypoglycemic effects. Herein, the effects of Imp on cell survival, carbohydrate-hydrolyzing enzymes (alpha-amylase and alpha-glucosidase), glucose uptake ability, insulin secretion levels, Advanced Glycation End products (AGEs) including pentosidine, methylglyoxal, and 3-deoxyglucosone levels and the activity of glyoxalase I as the main factor for degradation of AGEs were examined.<br /></span><span class="fontstyle0"><strong>Materials and Methods:</strong> </span><span class="fontstyle2">C2C12 skeletal muscle and beta-TC6 pancreatic cells were incubated with Imp at concentrations of 0, 25, 50, 75, and 10 μg/ml and the cells were evaluated separately. The biological assays were based on ultraviolet-visible (UV/VIS) spectrophotometric and/or high-performance liquid chromatography (HPLC) methods.<br /></span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">Imp had considerable and dose-dependent effects on glucose uptake and insulin secretion (<em>P</em><0.05). The highest levels of glucose uptake were achieved at a concentration of 100 μg/ml of Imp. Increased glycation index, cytotoxicity, and decreased glyoxalase I activity appeared mostly at concentrations of 75 μg/ml and higher. The studied alkaloid demonstrated remarkable hypoglycemic effect by inhibition of alpha-amylase and alpha-glucosidase.<br /></span><span class="fontstyle0"><strong>Conclusions:</strong> </span><span class="fontstyle2">Consequently, the results of the present study revealed possible hypoglycemic effects of Imp and it could be suggested for future studies in the treatment of diabetes mellitus.</span>  https://www.biotechrep.ir/article_125323_1af6e107b7c7730abb01794167b2098c.pdf