TY - JOUR ID - 69197 TI - Computer Aided Design of a Luciferase Like Haloalkane Dehalogenase Enzyme by Homology Based Rational Protein Design (HRPD) Method JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Satpathy, Raghunath AU - Konkimalla, V. Badireenath AU - Ratha, Jagnyeswar AD - School of Life science, Sambalpur University, Jyoti vihar, Burla, Odisha, India AD - School of Biological Sciences, National Institute of Science Education and Research (NISER), Bhubaneswar, Odisha, India Y1 - 2015 PY - 2015 VL - 2 IS - 4 SP - 315 EP - 323 KW - Protein Design KW - Chromophore KW - Luciferase KW - Haloalkane Dehalogenase KW - Docking KW - Mo-lecular Dynamics Simulation DO - N2 - Rational protein design is an important aspect to introduce novel characters to the enzymes. In this report, we describe a procedure for in-silico design of a novel haloalkane dehalogenase protein that exhibits luciferase property, which can be potentially used in biosensor applications. The haloalkane dehalogenase have a close structural homology with a lucifearse was used as the starting structure for design. Amino acids that are frequently interacts with the chromophre (colentrizine) was analyzed by docking and molecular dynamics simulation. The amino acids Asp170, Lys192, Arg193, Lys259, and Lys261 were selected in the enzyme structure for substitution purpose to generate mutant enzyme. Following several selection strategies, it was observed that the protein substituted with Phe in all the positions is the best one which was further validated by a 10 ns molecular dynamics simulation. The designed protein is then analyzed for its substrate specificity towards 10 selected toxic haloalkane compounds. The result shows, that the designed protein has also improved the substrate specificity towards four toxic pollutants. UR - https://www.biotechrep.ir/article_69197.html L1 - https://www.biotechrep.ir/article_69197_06a6b4d042c02067406af8a9a2f78a80.pdf ER -