TY - JOUR ID - 164652 TI - The Immunogenicity of a YncE Antigen from E. coli Enterotoxigenic (ETEC) by Edible Delivery of Transgenic Hairy Roots Tobacco JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Fatemeh, Mahmoodi Lamooki AU - Amani, Jafar AU - Motamedi, Mohammad Javad AU - Kazemi, Rouhollah AU - Shahriari Ahmadi, Farajollah AD - Department of Crop Biotechnology and Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran AD - Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Molecular Biology Research Center, Green Gene Company, Tehran, Iran Y1 - 2022 PY - 2022 VL - 9 IS - 4 SP - 856 EP - 863 KW - Diarrheal Disease KW - E. coli Enterotoxigenic KW - YncE Protein KW - Transgenic Tobacco KW - Hairy Roots KW - Oral Immunization DO - 10.30491/jabr.2021.248555.1285 N2 - Introduction: One of the most causes of diarrhea disease is Escherichia coli enterotoxigenic (ETEC). The first stage of the disease is the binding of ETEC to small intestinal epithelial cells by colonization factors. ETEC then produces Heat-labile enterotoxin (LT) and heat-stable (ST) enterotoxins. The yncE gene potentially encodes a protein with sequence similarity to a pyrroloquinoline quinone containing periplasmic oxidase. The YncE gene is conserved in various strains of E. coli. The protein encoded by the YncE gene is present in the membrane structure of the ETEC. The YncE gene can be considered as a novel protective vaccine candidate. The aim of the present study was to investigate herbal vaccines as a solution to health problems, especially in developing countries.Materials and Methods: In the present study, the expression of the YncE protein in tobacco hairy roots and its immunogenicity in mice were investigated. The YncE gene was cloned downstream of the CaMV-35S promoter in the binary expression vector, pBI121-YncE, by using different strains of the Agrobacterium rhizogenes (A4, MSU, 15834) and LBA4404. Three groups of mice including edible, edible-injection, and control were examined. ELISA method was used to determination of IgG and IgA in Feces and Serum.Results: The amount of the YncE protein was estimated approximately 0.9% of the Total Soluble Protein (TSP) in the transgenic hairy roots. The results indicate that the recombinant YncE protein produced in the transgenic hairy roots tobacco was able to stimulate the immune response in BALB/c mice. Also, it can be stated that recombinant YncE protein is an effective immunogen. The results implied the potential of transgenic tobacco hairy roots–based expression for oral-injection and oral delivery of YncE protein. The antibody titer showed that the immune system was well stimulated.Conclusions: The plant-based vaccine recombinant YncE protein could both humoral and mucosal the immune response, effectively. Therefore, the YncE antigen was able to stimulate the immune system of mice and produce antibodies. Actually, it appears to be a suitable vaccine candidate for ETEC-induced diarrhea.  UR - https://www.biotechrep.ir/article_164652.html L1 - https://www.biotechrep.ir/article_164652_f7dfb9697945b3e6deae5d4838a9cedf.pdf ER -