TY - JOUR ID - 156457 TI - Screening of Biotransformation Metabolites of α-Pinene by Two Bacterial strains Paenibacillus popilliae 1C, and Streptomyces rochei AB1 JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Saidani, Fatiha AU - Riad, Nacera AU - Beichi, Madjeda AU - Ferradji, Fatima Zohra AU - Zahi, Mohamed Reda AU - Edouaouda, Kamel AU - Mesbaiah, Fatma-Zohra AU - Bouzidi, Naima AU - Badis, Abdelmalek AU - El Hattab, Mohamed AD - Laboratory of Natural Products Chemistry and Biomolecules, Faculty of Sciences - University Blida 1, Road of Soumaa - BP 270- Blida 09000, Algeria Y1 - 2022 PY - 2022 VL - 9 IS - 3 SP - 763 EP - 774 KW - Biotransformation KW - α-pinene KW - Peanibacillus popilliae 1C KW - Streptomyces rochei AB1 KW - Chemical analysis DO - 10.30491/jabr.2021.288335.1396 N2 - Introduction: Biotransformation can be an effective tool for the structural modification of bioactive natural and synthetic compounds to synthesize novel and more potent compounds. The present study describes the biotransformation of α-Pinene using two bacterial strains Peanibacillus popilliae 1C and Streptomyces rochei AB1, isolated and identified in our previous study.Materials and Methods: The inhibitory concentration of the target substance against both strains was evaluated as 15 mg/ml. However, for technical considerations, a concentration of the α-Pinene at 10 mg/ml as Biotransformation Assimilable Concentration (BAC) was used to carry out the biotransformation process. For the biotransformation highlighting, both strains were cultivated on the rich medium (Luria-Bertani (LB) for Peanibacillus popilliae 1C and International Streptomyces Project 9 (ISP9) for Streptomyces rochei AB1) and poor medium (Minimum Medium (MM) for 1C and ISP9 for AB1). The chemical composition and percentage content of each compound were performed by GC/MS and GC/FID, respectively.Results: The GC/MS analysis revealed that all the biotransformation products were hydrocarbon and oxygenated monoterpenes, which can be divided into two groups. The first group of 11 compounds (Verbenene, Isocarveol, E-2,3-Epoxycarane, γ-Terpinene, Dehydrolinalool, α-Campholene aldehyde, Menthol, Carvacrol, Limonene dioxide, Piperitenone, Ocimenol) is described for the first time in the biotransformation of α-Pinene and a second group bringing together 8 compounds previously reported (Trans Verbenol, p-Cymen-8-ol, α-Terpineol, Myrtenol, Verbenone, Trans Sobrerol, D-Limonene, Pinocarvone).Conclusions: These bacterial strains possess distinctive biocatalytic capacities toward α-Pinene which leads to the production of other secondary metabolites.  UR - https://www.biotechrep.ir/article_156457.html L1 - https://www.biotechrep.ir/article_156457_d38bf3ee4b2fee72a38e1f2723af17d7.pdf ER -