TY - JOUR ID - 151758 TI - Isolation and Purification of Antifungal Compounds from the Green Microalga Chlorella vulgaris JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Sultan, Yousef Y. AU - Marrez, Diaa A. AD - Food Toxicology and Contaminants Department, National Research Centre, Cairo, Egypt Y1 - 2022 PY - 2022 VL - 9 IS - 2 SP - 603 EP - 613 KW - Chlorella vulgaris KW - Fractions KW - Antifungal KW - Mycotoxigenic Fungi KW - Bioautography DO - 10.30491/jabr.2021.302307.1438 N2 - Introduction: The current study aimed to purify antifungal compounds from Chlorella vulgaris extracts, fractions, sub-fractions and pure compounds against different strains of mycotoxigenic fungi.Materials and Methods: Antifungal activity was conducted using disc diffusion assay, TLC-bioautography and Minimum Inhibitory Concentration (MIC). Isolation, purification and structure elucidation of antifungal compounds were carried out using column chromatography, Thin Layer Chromatography (TLC), UV-Vis spectrophotometer, Gas Chromatography–Mass Spectrometry (GC-MS), and Nuclear Magnetic Resonance (NMR).Results: C. vulgaris Diethyl Ether Extract (DEE) showed the highest antifungal activity against all tested fungi with inhibition zone from 11.5 to 21.9 mm. By fractionation of DEE, Fraction F3 (chloroform:methanol, 50:50) and F5 (methanol 100%); sub-fraction CF3-10 and CF5-10 exhibited antifungal activity against all tested fungi. Two pure compounds, hydroxyphenophytin B and hexadecanoic acid methyl ester, with antifungal activity were isolated from CF3-10 and CF5-10, respectively.Conclusions: C. vulgaris DEE and isolated compounds can be used as promising antifungal agents from natural sources against mycotoxigenic fungi at post-harvest or storage stages.  UR - https://www.biotechrep.ir/article_151758.html L1 - https://www.biotechrep.ir/article_151758_41c88dc30ee90ed65057a821864933cf.pdf ER -