TY - JOUR ID - 143057 TI - Molecular Characterization and Phenotype Analysis of a Novel Psychrotrophic Exiguobacterium Species from the Ilam Mountains JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Nasrollahzadeh Sabet, Mehrdad AU - Akbariqomi, Mostafa AU - Rigi, Garshasb AU - Saberian, Ali AU - Behroozi, Javad AU - Heidari, Mohammad Foad AU - Elmi, Taher AU - Alimohamadi, Fereshteh AU - Heidari, Reza AD - School of Medicine, AJA University of Medical Sciences, Tehran, Iran AD - Applied Biotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Department of Genetics, Faculty of Basic Science, Shahrekord University, Rahbar Blvd, Shahrekord, Iran AD - Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord 88137-33395, Iran AD - Cancer Epidemiology Research and Treatment Center, AJA University of Medical Sciences, Tehran, Iran AD - Department of Medical Laboratory Sciences, School of Allied Health Medicine, AJA University of Medical Sciences, Tehran, Iran AD - Department of Laboratory Science, Babol Branch, Islamic Azad University, Babol, Iran Y1 - 2021 PY - 2021 VL - 8 IS - 4 SP - 440 EP - 452 KW - Psychrotrophic KW - Genus Exiguobacterium KW - Phenotype Analysis KW - R packages KW - Data Analysis DO - 10.30491/jabr.2022.308621.1453 N2 - Introduction: The genus Exiguobacterium includes psychrotrophic, mesophilic, and moderate thermophilic strains and species that have been isolated from extreme environments, including very cold or hot environments. The genus Exiguobacterium has received much attention in biotechnology, and related industries due to secretory enzymes with high enzymatic activity and consequently stable enzymes capable of tolerating extreme conditions. The aim of the present study was to introduce and describe the phenotypic and the genotypic characterization of a novel species of genus Exiguobacterium isolated from the Ilam mountains, Iran.Materials and Methods: Genotypic features were analyzed using universal genes (gyrB, hsp70, rpoB, and citC) belonging to the Exiguobacterium genus. Also, cspC1, nusA, and dnaJ genes were used to confirm the profile of new psychrotrophic strains. The distinctive phenotypic characteristics of the new strain with the most famous strains of the genus Exiguobacterium were investigated. All statistical analyses were conducted using R Packages for data visualization and exploratory data.Results: A motile, Gram-stain-positive, rod-shaped, non-sporing, tolerant up to 5% NaCl, grew at 0-25 °C and pH 6 and 9, designated Exiguobacterium sp. HA2 was isolated from the soil. The major isoprenoid quinone is MK-7 and in the smaller amount are MK-6 and MK-8. The major cellular fatty acids (>10 %) were isoC13:0, isoC15:0, and C16:0. To adapt to low temperatures, Exiguobacterium sp. HA2 upregulated expresses cold shock response including cold shock protein (CSP) and transcription elongation protein NusA. Also, downregulated expression of heat shock protein DnaJ domain protein.Conclusions: In the current study we investigated the difference in the genotype, phenotypic, and functional characteristics of Exiguobacterium sp. HA2. It can be regarded as representing a novel psychrotrophic species within the genus Exiguobacterium.  UR - https://www.biotechrep.ir/article_143057.html L1 - https://www.biotechrep.ir/article_143057_37156662114675e7158609f7a3e1810f.pdf ER -