TY - JOUR ID - 138075 TI - Regeneration of Sciatic Nerve Injury by Polyglycolic Acid/Collagen/Bioglass Conduit JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Dehnavi, Navid AU - Parivar, Kazem AU - Goodarzi, Vahabodin AU - Salimi, Ali AU - Mansoori, Kourosh AU - Nourani, Mohammad Reza AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran AD - Neuromusculoskletal Research Centre Firozgar Hospital, Iran University of Medical Science, Tehran, Iran Y1 - 2021 PY - 2021 VL - 8 IS - 3 SP - 283 EP - 292 KW - Biomaterial KW - bioglass KW - Nerve Regeneration KW - Polyglycolic Acid KW - Tissue engineering DO - 10.30491/jabr.2020.227237.1213 N2 - Introduction: The repair process of severed peripheral nerves is conducted by the bridging of the regenerating neural fibers across a gap in the case of the existence of an appropriate route (space) between the proximal and distal severed stumps. The current study aimed to improve the transected sciatic nerve of rats with a 10 mm gap by means of applying electrospun conduits composed of polymer nanocomposites of polyglycolic acid (PGA), collagen, and nanobioglass (NBG). Then, the efficacy of the designed conduits (PGA/collagen/NBG, PGA/collagen, and PGA alone) was histologically and electrophysiologically compared with autograft nerves to determine whether these conduits have superiority over the autograft procedures in the process of nerve regeneration.Materials and Methods: In this experiment, 50 healthy adult male Wistar rats underwent sciatic nerve transection. After four, eight, and 12 weeks of the surgical procedures, the therapeutic effects of conduits on sensory and motor recovery of transected nerves were evaluated.Results: The analysis of the functions of motor and sensory nerves showed marked improvement in rats treated with PGA/collagen/NBG conduit. Also, histological staining and immunohistochemical assessment of the expression of NF200, S100, and CD31 proteins revealed newly-formed nerve fibers with micro blood vessels at the proximity of regenerated nerve fibers.Conclusions: It seems that due to the high surface area of electrospun nerve conduits to adhere the cells, the application of these compounds would be beneficial in clinical practice in the future. The results suggest that PGA/Collagen/NBG nanofibrous conduit possesses the highest capability in increasing nerve regeneration following nerve transection in murine models. UR - https://www.biotechrep.ir/article_138075.html L1 - https://www.biotechrep.ir/article_138075_cb0449ae3a6cc21b80fd3ce9818b79a4.pdf ER -