TY - JOUR ID - 131084 TI - An Insight Into SARS-CoV-2 Phylogenetics and Genomics for Sixty Isolates Occurring in India JO - Journal of Applied Biotechnology Reports JA - JABR LA - en SN - 2322-1186 AU - Reddy Gujjula, Koteswara AU - Reddy Varakala, Nikhil AU - Dhakate, Divyanshu AU - Ellamla, Harikishan R. AU - Jabes B, Shadrack AD - Department of Biotechnology, Koneru Lakshmaiah Education Foundation (Deemed to be University), Green Fields, Vaddeswaram-522502, Guntur, Andhra Pradesh, India AD - Eduard-Zintl-Institut für Anorganische und Physikalische Chemie, Centre of Smart Interfaces, Technische Universität Darmstadt, Alarich-Weiss-Straße 10, 64287, Darmstadt, Germany AD - Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luiz, São Carlos-13565905, Brazil Y1 - 2021 PY - 2021 VL - 8 IS - 2 SP - 116 EP - 126 KW - SARS-CoV-2 KW - COVID19 KW - Phylogenetics KW - Genomics KW - Motif KW - Vaccine target DO - 10.30491/jabr.2021.260175.1319 N2 - Introduction: Analysis of genome sequences to search for encoded proteins and motifs is the most widely used technique for the prediction of new drug and vaccine targets. It can effectively leverage computational techniques to deliver effective and pragmatic advantages in the search for new drugs and vaccines. Materials and Methods: We examine the diversity and evolution of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) isolates from different geographical parts of India using phylogenetic tree analysis. A dataset of 172 Indian SARS-CoV-2 genome sequences was collected from a database and a phylogenetic tree was constructed. Results: From the phylogenetic analysis, we identified 6 different clusters and from each cluster, we have chosen 10 genome sequences to find open reading frames (ORFs) and common encoded proteins. We found 4 encoded proteins that are common among the 60 genome sequences and they correspond to ORF7a protein, membrane glycoprotein, surface glycoprotein, and nucleocapsid phosphoproteins. Our results highlight that there are 6 conserved motifs with a high frequency of occurrence suggesting that potentially use in further study. Conclusions: The encoded proteins and their detected sequential motifs might be useful for screening potential drugs and vaccine candidates of SARS-CoV-2 Indian isolates in the current epidemic situation. UR - https://www.biotechrep.ir/article_131084.html L1 - https://www.biotechrep.ir/article_131084_b78df9db3b3017303dc8d99af1379983.pdf ER -