%0 Journal Article %T Electrochemical Behavior of Adult and Fetal Hemoglobin at Gold-coated Magnetic Iron Oxide Nanoparticle %J Journal of Applied Biotechnology Reports %I Baqiyatallah University of Medical Sciences %Z 2322-1186 %A Eskandari, Khadijeh %A Ghourchian, Hedayatollah %A Banaei, Aghdas %D 2017 %\ 09/01/2017 %V 4 %N 3 %P 669-674 %! Electrochemical Behavior of Adult and Fetal Hemoglobin at Gold-coated Magnetic Iron Oxide Nanoparticle %K Biosensor %K Direct Electrochemistry %K H2O2 %K Gold-coated %K Iron Oxide Nanoparticles %K Hemoglobin %R %X A feasible and fast method for Adult Hemoglobin (A-Hb) and Fetal Hemoglobin (F-Hb) study was developed by immobilization of Hb on gold-coated magnetic iron oxide nanoparticles (GMNPs).The prepared GMNPs composite nanoparticles with 60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The direct electroche- mistry of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corres- ponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (α) for electron transfer between the electrode surface and pro- tein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb biosensors for H O detection. The lifetime of biosensor is more than 2 weeks. %U https://www.biotechrep.ir/article_68968_6f5f0de10c12f0a71c9e72c6dfa307e5.pdf