@article { author = {Eskandari, Khadijeh and Ghourchian, Hedayatollah and Banaei, Aghdas}, title = {Electrochemical Behavior of Adult and Fetal Hemoglobin at Gold-coated Magnetic Iron Oxide Nanoparticle}, journal = {Journal of Applied Biotechnology Reports}, volume = {4}, number = {3}, pages = {669-674}, year = {2017}, publisher = {Baqiyatallah University of Medical Sciences}, issn = {2322-1186}, eissn = {2423-5784}, doi = {}, abstract = {A feasible and fast method for Adult Hemoglobin (A-Hb) and Fetal Hemoglobin (F-Hb) study was developed by immobilization of Hb on gold-coated magnetic iron oxide nanoparticles (GMNPs).The prepared GMNPs composite nanoparticles with 60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The direct electroche- mistry of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corres- ponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (α) for electron transfer between the electrode surface and pro- tein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb biosensors for H O detection. The lifetime of biosensor is more than 2 weeks.}, keywords = {Biosensor,Direct Electrochemistry,H2O2,Gold-coated,Iron Oxide Nanoparticles,Hemoglobin}, url = {https://www.biotechrep.ir/article_68968.html}, eprint = {https://www.biotechrep.ir/article_68968_6f5f0de10c12f0a71c9e72c6dfa307e5.pdf} }