@article { author = {Rashidiani, Jamal and Eskandari, Khadijeh and Ranjbar, Reza and Kooshki, Hamid and Afshar, Davoud and Safarpoor Dehkordi, Farhad}, title = {Application of Gold Core-Shell Magnetic Nanoparticles as an Immunosensor for the Detection of Vibrio cholerae}, journal = {Journal of Applied Biotechnology Reports}, volume = {8}, number = {1}, pages = {-}, year = {2021}, publisher = {Baqiyatallah University of Medical Sciences}, issn = {2322-1186}, eissn = {2423-5784}, doi = {10.30491/jabr.2020.230689.1222}, abstract = {Introduction: For rapid and sensitive detection of Vibrio cholerae an accurate assay is needed. In the present study, a gold-coated magnetic nanoparticle (GMNP) was investigated for the detection of V. cholerae. Materials and Methods: GMNPs were synthesized and functionalized by 11-mercapto-undecanoic acid (MUA) as a linker for immobilization of IgG against V. cholerae OmpW antigen. In the next step, IgG was coupled with carboxylic group of MUA using EDC / NHS and the IgG/GMNPs nanocomposite created and finally, the bacterium was detected in a sandwich model ELISA. Results: The IgG/GMNPs nanocomposite could detect V.cholerae in a concentration range from 2.5×10-2 to 1.5 × 105 N/ml (number of V.cholerae per ml). The correlation coefficient was 0.99 and the detection limit was 16 N/ml. Conclusions: In this study, the procedure of antibody immobilization on magnetic nanoparticles was designed. So that, by using magnetic nanoparticles, the pre-concentration as a time-consuming step was removed and the sensitivity of V. cholerae determination was increased. Also, this method can be extended to detect other microorganisms.}, keywords = {Vibrio cholerae,Gold,Magnetic nanoparticles,Detection}, url = {https://www.biotechrep.ir/article_120188.html}, eprint = {https://www.biotechrep.ir/article_120188_bcb7f424fcc5bc7aefb17c0015b1bf39.pdf} }