@article { author = {Hosseini, Mostafa and Ebrahimi, Morvarid and Salehghamari, Ensieh and Salehi Najafabadi, Amir and Yakhchali, Bagher}, title = {Biotransformation of Isobutyraldehyde to Isobutanol by an Engineered Escherichia coli Strain}, journal = {Journal of Applied Biotechnology Reports}, volume = {7}, number = {3}, pages = {159-165}, year = {2020}, publisher = {Baqiyatallah University of Medical Sciences}, issn = {2322-1186}, eissn = {2423-5784}, doi = {10.30491/jabr.2020.117885}, abstract = {Introduction: Biotransformation process has been used in various industries due to its ability to produce valuable chemicals and address environmental concerns. Propylene hydroformylation is a process in which n-butyraldehyde and isobutyraldehyde are produced. N-butyraldehyde is a high valuable chemical with many industrial applications, while isobutyraldehyde produced as a by-product is an environmental pollutant. This study offers a biotechnological approach for conversion of isobutyraldehyde into a high-value substance. An engineered strain of Escherichia coli was developed by genomic insertion of alcohol-dehydrogenase gene (adhA) from Lactococcus lactis which can convert isobutyraldehyde into isobutanol.Materials and Methods: The adhA gene was engineered to substitute some of its amino acids to result in a more efficient enzyme. Engineered gene was synthesized and introduced into E. coli genome to develop recombinant E. coli EG-296 strain. In addition, by using the Qualiteck-4 software, 16 well-defined experiments (L16 Orthogonal array) with two levels of seven variable parameters were used to optimize the process efficiency.Results: The findings of this study revealed that the E. coli strain EG-296 is capable of converting isobutyraldehyde into isobutanol. The optimization results showed that optimum medium composition for the highest isobutanol production were 10 g/L glucose or glycerol as carbon source, 10 g/L NH4CL as nitrogen source, mid-log of inoculum age, and 1% inoculum volume in 25ml medium. After optimization, 560 mg/L isobutanol was produced from 600 mg/L isobutyraldehyde with 91% yield.Conclusions: Recombinant E. coli strain with a relatively optimum medium can be used to remove isobutyraldehyde in refineries or other industries producing this chemical as a by-product.}, keywords = {Biotransformation,Isobutyraldehyde,Recombinant Escherichia coli,Isobutanol,optimization}, url = {https://www.biotechrep.ir/article_117885.html}, eprint = {https://www.biotechrep.ir/article_117885_a2b01ae8300621b2f8bce44a1d6222b6.pdf} }