Journal of Applied Biotechnology Reports

Journal of Applied Biotechnology Reports

Optimization of Catalase Production by Kocuria sp. ASB107 Using Response Surface Methodology and Molasses as the Carbon Source

Document Type : Original Article

Authors
Elham Godini
Ezat Asgarani
Jamshid Fooladi
Tahereh Ghashghaei
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract
Catalase is an antioxidant enzyme that has various applications in different industries, such as food, dairy, textile, etc. Alkaline catalase is suitable in this regard. Kocuria sp. ASB107 is a radio-resistant bacterium that has been isolated from Ab-e-siahspring in Ramsar (Mazandaran Province, Iran). This bacterium is able to produce relative high amounts of alkaline catalase and hence this catalase has the potential for industrial applications. Production of enzyme in a short time and with low expenses is of a great importance.  Lag phase of Kocuria sp. ASB107 growth takes a long time in TSB medium (about 13 hours).  In order to achieve the highest rate of bacterial growth, 5% inoculum size of cells was used in the middle logarithmic phase and as a result, bacterial lag phase decreased to 2 hours. On the other hand, for economic production of catalase, sugar beet molasses was used as a cheap carbon source. Several factors effecting bacterial growth and enzyme activity were selected for the optimization of catalase production by Kocuria sp. ASB107 using Response Surface Methodology (RSM) based on the Central Composite Design (CCD).  Four variables (carbon source, nitrogen source, agitation, and inoculum size) were selected for the optimization studies. Results showed that Kocuria sp. ASB107 exhibits optimum rate of catalase activity (3399.08 U/ml) and bacterial biomass (6.904 g/L) with carbon source 3% v/v, nitrogen source 0.5% w/v, inoculum size 7.5% v/v, and agitation 200 rpm.
Keywords
Catalase
Kocuria sp. ASB107
optimization
RSM
Sugar Beet Molasses
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Journal of Applied Biotechnology Reports
Volume 4, Issue 1
Winter 2017
Pages 519-525

  • Receive Date 17 February 2017
  • Revise Date 25 March 2017
  • Accept Date 25 March 2017