Screening of Biotransformation Metabolites of α-Pinene by Two Bacterial strains Paenibacillus popilliae 1C, and Streptomyces rochei AB1

Saidani, Fatiha; Riad, Nacera; Beichi, Madjeda; Ferradji, Fatima Zohra; Zahi, Mohamed Reda; Edouaouda, Kamel; Mesbaiah, Fatma-Zohra; Bouzidi, Naima; Badis, Abdelmalek; El Hattab, Mohamed

doi:10.30491/jabr.2021.288335.1396

Screening of Biotransformation Metabolites of α-Pinene by Two Bacterial strains Paenibacillus popilliae 1C, and Streptomyces rochei AB1

Document Type : Original Article

Authors

Fatiha Saidani ¹

Nacera Riad ¹

Madjeda Beichi ¹

Fatima Zohra Ferradji ¹

Mohamed Reda Zahi ¹

Kamel Edouaouda ¹

Fatma-Zohra Mesbaiah ¹^{, 2}

Naima Bouzidi ¹

Abdelmalek Badis ¹

Mohamed El Hattab ¹

¹ Laboratory of Natural Products Chemistry and Biomolecules, Faculty of Sciences - University Blida 1, Road of Soumaa - BP 270- Blida 09000, Algeria

² National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), BouIsmaïl, 42415, Tipaza, Algeria

10.30491/jabr.2021.288335.1396

Abstract

Introduction: Biotransformation can be an effective tool for the structural modification of bioactive natural and synthetic compounds to synthesize novel and more potent compounds. The present study describes the biotransformation of α-Pinene using two bacterial strains Peanibacillus popilliae 1C and Streptomyces rochei AB1, isolated and identified in our previous study.
Materials and Methods: The inhibitory concentration of the target substance against both strains was evaluated as 15 mg/ml. However, for technical considerations, a concentration of the α-Pinene at 10 mg/ml as Biotransformation Assimilable Concentration (BAC) was used to carry out the biotransformation process. For the biotransformation highlighting, both strains were cultivated on the rich medium (Luria-Bertani (LB) for Peanibacillus popilliae 1C and International Streptomyces Project 9 (ISP9) for Streptomyces rochei AB1) and poor medium (Minimum Medium (MM) for 1C and ISP9 for AB1). The chemical composition and percentage content of each compound were performed by GC/MS and GC/FID, respectively.
Results: The GC/MS analysis revealed that all the biotransformation products were hydrocarbon and oxygenated monoterpenes, which can be divided into two groups. The first group of 11 compounds (Verbenene, Isocarveol, E-2,3-Epoxycarane, γ-Terpinene, Dehydrolinalool, α-Campholene aldehyde, Menthol, Carvacrol, Limonene dioxide, Piperitenone, Ocimenol) is described for the first time in the biotransformation of α-Pinene and a second group bringing together 8 compounds previously reported (Trans Verbenol, p-Cymen-8-ol, α-Terpineol, Myrtenol, Verbenone, Trans Sobrerol, D-Limonene, Pinocarvone).
Conclusions: These bacterial strains possess distinctive biocatalytic capacities toward α-Pinene which leads to the production of other secondary metabolites.

Keywords

Biotransformation

α-Pinene

Peanibacillus popilliae 1C

Streptomyces rochei AB1

Chemical Analysis